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Biotechnology Information ncbi primer blast primer 3 software
Ncbi Primer Blast Primer 3 Software, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+blast+software/blast+primer/10__1016_slash_j__scienta__2026__114754-106-6-16
Average 86 stars, based on 1 article reviews
ncbi primer blast primer 3 software - by Bioz Stars, 2026-11
86/100 stars

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Article Title: Network pharmacology and molecular dynamics simulation elucidate the potential mechanism of Batatasin-III in Bletilla striata against ulcerative colitis.
Article Snippet: .. For primers designing online Primer-BLAST software from the National Center for Biotechnology Information (NCBI) was employed (Table 1). .. Shanghai Sangon Biotech Co., Ltd. synthesized the primers.

Article Title: Network pharmacology and molecular dynamics simulation elucidate the potential mechanism of Batatasin-III in Bletilla striata against ulcerative colitis
Article Snippet: .. For primers designing online Primer-BLAST software from the National Center for Biotechnology Information (NCBI) was employed (Table ). .. Shanghai Sangon Biotech Co., Ltd. synthesized the primers.

Article Title: LysM Receptor Proteins are Required for Ectomycorrhizal Symbiosis in Poplar
Article Snippet: The qRT-PCR was performed on the BioRad C1000Touch CFX96 system with the following program: initial denaturation step 95 °C for 15 min, followed by repeated cycles of denaturation, annealing, and extension as follows; 39 cycles of 95 °C for 15 s, 58 °C for 30 s. A melt curve analysis was performed to assess the specificity of the PCR product at 95 °C for 10 s, 90 °C for 5s. .. Primers used in this study (Table S1) were designed with the Primer-BLAST software (National Center for Biotechnology Information) Analysis of the resulting data was implemented by delta-Ct (2^−ΔCT) method ( ). .. Statistical analyses were performed with R-Studio (version 3.2.4) Significance of results was determined by ANOVA and posthoc Tukey test.

Article Title: Acid-sensing ion channel 3 in macrophages, but not sensory neurons, mediates development of activity-induced muscle pain.
Article Snippet: Activity-induced pain is a significant symptom for people with chronic pain and reduces participation in daily activities and effective exercise programs.. We previously showed that pharmacological blockade of ASIC3 locally in muscle, but not downregulation of ASIC3 in sensory neurons, prevents development of activity-induced muscle pain.. Further, depleting macrophages from muscle prevents activity-induced pain.

Article Title: Brown Algae Extracts Increase the Tolerance of Tomato Plants to High Temperatures by Improving Morphological, Physiological, Metabolomic, and Transcriptional Parameters
Article Snippet: The primers were actin as an endogenous gene ( ACT ) and nine study genes: NCED1 (9-cis-expoxycarotenoid dioxygenase 1), HSP70 (heat shock protein 70), PIP2 (plasma membrane intrinsic protein; aquaporin 2), P5CS1 (delta1-pyrroline-5-carboxylate synthase 1), ERD15 (protein of early response to dehydration 15), Fe-SOD (iron superoxide dismutase), CAT1 (catalase 1), cAPX2 (cytosolic ascorbate peroxidase 2), and PAL5-3 (phenylalanine ammonia lyase 5-3). .. The primers were designed using Primer BLAST software ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHome , National Center for Biotechnology Information NCBI, Bethesda, USA) and Oligoanalyzer 3.1 (Integrated DNA Technologies IDT, Coralville, IA, USA), except for ERD15 , Fe-SOD , and cAPX2 , which were obtained from Ziaf et al. [ ] and Mascia et al. [ ], respectively. ..

Article Title: Control of alkaline phosphatase activity and pH stability by taurine in liquid boar semen
Article Snippet: .. The primer sequences for target genes of outer dense fiber of sperm tails protein 2 (ODF2), zona pellucida binding protein 2 (ZPBP2), and A-kinase anchor proteins 3 (AKAP3) and 4 (AKAP4) were designed using Primer-BLAST software from the National Center for Biotechnology Information (NCBI), Bethesda, USA. ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ) ( ). .. The experimental data were displayed as mean ± SEM and were statistically analyzed using one-way ANOVA in GraphPad PRISM (GraphPad Software).

Article Title: Brown Algae Extracts Increase the Tolerance of Tomato Plants to High Temperatures by Improving Morphological, Physiological, Metabolomic, and Transcriptional Parameters.
Article Snippet: The primers were actin as an endogenous gene (ACT) and nine study genes: NCED1 (9-cis-expoxycarotenoid dioxygenase 1), HSP70 (heat shock protein 70), PIP2 (plasma membrane intrinsic protein; aquaporin 2), P5CS1 (delta1-pyrroline-5carboxylate synthase 1), ERD15 (protein of early response to dehydration 15), Fe-SOD (iron superoxide dismutase), CAT1 (catalase 1), cAPX2 (cytosolic ascorbate peroxidase 2), and PAL5-3 (phenylalanine ammonia lyase 5-3). .. The primers were designed using Primer BLAST software (https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_ LOC=BlastHome, National Center for Biotechnology Information NCBI, Bethesda, USA) and Oligoanalyzer 3.1 (Integrated DNA Technologies IDT, Coralville, IA, USA), except for ERD15, Fe-SOD, and cAPX2, which were obtained from Ziaf et al. [65] and Mascia et al. [66], respectively. ..

Article Title: Network pharmacology and experimental validation to elucidate the pharmacological mechanisms of Guben Xiezhuo decoction against renal fibrosis
Article Snippet: .. For primers designing online Primer-BLAST software from the National Center for Biotechnology Information (NCBI) was employed (Table ). .. Shanghai Sangon Biotech Co., Ltd. synthesized the primers.

Real-time Polymerase Chain Reaction:

Article Title: Acid-sensing ion channel 3 in macrophages, but not sensory neurons, mediates development of activity-induced muscle pain.
Article Snippet: Activity-induced pain is a significant symptom for people with chronic pain and reduces participation in daily activities and effective exercise programs.. We previously showed that pharmacological blockade of ASIC3 locally in muscle, but not downregulation of ASIC3 in sensory neurons, prevents development of activity-induced muscle pain.. Further, depleting macrophages from muscle prevents activity-induced pain.

Binding Assay:

Article Title: Control of alkaline phosphatase activity and pH stability by taurine in liquid boar semen
Article Snippet: .. The primer sequences for target genes of outer dense fiber of sperm tails protein 2 (ODF2), zona pellucida binding protein 2 (ZPBP2), and A-kinase anchor proteins 3 (AKAP3) and 4 (AKAP4) were designed using Primer-BLAST software from the National Center for Biotechnology Information (NCBI), Bethesda, USA. ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ) ( ). .. The experimental data were displayed as mean ± SEM and were statistically analyzed using one-way ANOVA in GraphPad PRISM (GraphPad Software).



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